Barclays PLC and four former executives have been charged with conspiracy to commit fraud and the provision of unlawful financial assistance reports BBC News
The Serious Fraud Office charges come at the end of a five-year investigation and relate to the bank's fundraising at the height of 2008's financial crisis.
Former chief executive John Varley is one of the four ex-staff who will face Westminster magistrates on 3 July.
Barclays says it is considering its position and awaiting further details.
It added: "The charges arise in the context of Barclays' capital raisings in June and November 2008. Barclays awaits further details of the charges from the SFO."
Mr Varley, former senior investment banker Roger Jenkins, Thomas Kalaris, a former chief executive of Barclays' wealth division, and Richard Boath, the ex-European head of financial institutions, have all been charged with conspiracy to commit fraud in the June 2008 capital raising.
In addition, Mr Varley and Mr Jenkins have also been charged with the same offence in relation to the October 2008 capital raising and with providing unlawful financial assistance.
Mr Jenkins will "vigorously defend" himself against the charges, his lawyer has told Reuters.
"As one might expect in the challenging circumstances of 2008, Mr Jenkins sought and received both internal and external legal advice on each and every topic covered by the SFO's accusations," said Brad Kaufman fromNow, along with the bank he once led, he faces two types of charges, both of which relate to fundraisings from Qatar in 2008.
The first charge, conspiracy to commit fraud, relates to "advisory" fees paid to Qatar. The second - "unlawful assistance" - could be more serious.
It relates to a £2bn loan advanced to Qatar after the fundraisings were negotiated - the implication being that there was a money-go-round at work - Barclays was handing Qatar some of the money it was using to support the British bank.
Mr Varley was one of Britain's leading bankers, having been chief executive at Barclays for six years.
Barclays took £7bn from Qatar in 2008, as banks scrambled to avoid nationalisation. The SFO looked into payments made to Qatar at the same time and after the deal, and whether those payments were correctly disclosed, and whether those might have been inducements to Qatar to support the British bank.
The emergency funds allowed Barclays to avoid a government bailout in 2008 at a time when rivals Lloyds Banking Group and Royal Bank of Scotland were forced to rely on a taxpayer rescue.
The Financial Conduct Authority (FCA) has also reopened its probe into the deal and is understood to be reviewing new evidence which could prompt it to reconsider a £50m fine against the banking giant four years ago.
The FCA imposed the penalty after finding that Barclays had failed to disclose the arrangements and fees it paid to the Qatari investors, but Barclays contested the fine and it was put on hold while the SFO conducted its investigation.
The emergency funds raised by Barclays allowed it to avoid a government bailout in 2008 at a time when rivals Lloyds Banking Group and Royal Bank of Scotland were forced to rely on a taxpayer rescue. American firm Greenberg Traurig.